Questions to Ministers 1. CRAIG FOSS to the Minister of Finance: What reports has he received on the economy moving away from government spending, housing speculation and borrowing, and towards savings and exporting? 2. Hon PETE HODGSON to the Prime Minister: Does he stand by his answer to question No 11 yesterday that Sammy Wong did not accompany Pansy Wong on any ministerial trips to China? 3. HONE HARAWIRA to the Associate Minister of Health: What is the rationale behind the move to prohibit the display of tobacco products for sale in retail outlets? 4. Hon DARREN HUGHES to the Minister of Transport: Has he received any feedback or information in support of lowering the adult blood alcohol concentration to 0.05 that has given him any doubt or cause for reflection this year about his decision to retain the level at 0.08? 5. TIM MACINDOE to the Minister of Housing: What recent announcement has he made about the direction of social housing in New Zealand? 6. BRENDON BURNS to the Minister for Canterbury Earthquake Recovery: Is the Government's response package, announced last week to assist Canterbury businesses affected by the 4 September earthquake, universally supported by the Government and its supporters? 7. CATHERINE DELAHUNTY to the Minister for Social Development and Employment: Does she agree with the Alternative Welfare Working Group that welfare reform should be focused on "the relentless pursuit of well-being"? 8. Hon NANAIA MAHUTA to the Minister of Energy and Resources: Will the mining of lignite form part of his New Zealand Energy Strategy? 9. LOUISE UPSTON to the Minister of Education: What were the results of the Programme for International Assessment (PISA), which looked at New Zealand students' achievement in reading, maths and scientific literacy? 10. DARIEN FENTON to the Minister of Transport: How many emails has he received on the decision of the New Zealand Transport Agency to start charging CarJam and similar web-based information services for accessing the Agency's stored basic motor vehicle information? 11. JACQUI DEAN to the Minister of Agriculture: What recent steps has the Government taken to control bovine TB? 12. Hon TREVOR MALLARD to the Minister of Education: Which Minister is to be responsible for the enforcement of the Education Act 1989 in relation to limited attendance early childhood centres following the passing of the Education Amendment Bill (No 2)? Questions to Members 1. Hon DAVID CUNLIFFE to the Chairperson of the Finance and Expenditure Committee: How many submissions have been received on the Student Loan Scheme Bill? 2. Hon DAVID CUNLIFFE to the Chairperson of the Finance and Expenditure Committee: How many submitters on the Student Loan Scheme Bill have requested to be heard in person? 3. CHRIS HIPKINS to the Chairperson of the Local Government and Environment Committee: How many submissions have been received on the Sustainable Biofuel Bill? 4. CHRIS HIPKINS to the Chairperson of the Local Government and Environment Committee: How many submitters on the Sustainable Biofuel Bill have requested to be heard in person? 5. CHRIS HIPKINS to the Chairperson of the Local Government and Environment Committee: How many submissions have been received on the Southland District Council (Stewart Island/Rakiura Visitor Levy) Empowering Bill? 6. CHRIS HIPKINS to the Chairperson of the Local Government and Environment Committee: How many submitters on the Southland District Council (Stewart Island/Rakiura Visitor Levy) Empowering Bill have requested to be heard in person? 7. CHRIS HIPKINS to the Chairperson of the Government Administration Committee: How many submissions have been received on the Identity Information Confirmation Bill? 8. CHRIS HIPKINS to the Chairperson of the Government Administration Committee: How many submitters on the Identity Information Confirmation Bill have requested to be heard in person? 9. Hon PAREKURA HOROMIA to the Chairperson of the Māori Affairs Committee: How many submissions have been received on the Marine and Coastal Area (Takutai Moana) Bill? 10. Hon PAREKURA HOROMIA to the Chairperson of the Māori Affairs Committee: How many submitters on the Marine and Coastal Area (Takutai Moana) Bill have requested to be heard in person? 11. Hon DAVID CUNLIFFE to the Chairperson of the Finance and Expenditure Committee: Has the Finance and Expenditure Committee conducted the 2009/10 financial review of the Office of the Retirement Commissioner? 12. DARIEN FENTON to the Chairperson of the Transport and Industrial Relations Committee: How many submissions have been received on the Land Transport (Driver Licensing) Amendment Bill? 13. DARIEN FENTON to the Chairperson of the Transport and Industrial Relations Committee: How many submitters on the Land Transport (Driver Licensing) Amendment Bill have requested to be heard in person? 14. DARIEN FENTON to the Chairperson of the Transport and Industrial Relations Committee: How many submissions have been received on the Land Transport (Road Safety and Other Matters) Amendment Bill? 15. Hon DAVID PARKER to the Chairperson of the Justice and Electoral Committee: How many submissions have been received on the Lawyers and Conveyancers Amendment Bill? 16. Hon DAVID PARKER to the Chairperson of the Justice and Electoral Committee: How many submitters on the Lawyers and Conveyancers Amendment Bill have requested to be heard in person? 17. Hon DAVID PARKER to the Chairperson of the Justice and Electoral Committee: How many submissions have been received on the Legal Services Bill? 18. Hon DAVID PARKER to the Chairperson of the Justice and Electoral Committee: How many submitters on the Legal Services Bill have requested to be heard in person? 19. Hon DAVID PARKER to the Chairperson of the Justice and Electoral Committee: How many submissions have been received on the Sale and Supply of Liquor and Liquor Enforcement Bill? 20. Hon DAVID PARKER to the Chairperson of the Justice and Electoral Committee: How many submitters on the Sale and Supply of Liquor and Liquor Enforcement Bill have requested to be heard in person? 21. Hon DAMIEN O'CONNOR to the Chairperson of the Primary Production Committee: How many submissions have been received on the Food Bill? 22. Hon DAMIEN O'CONNOR to the Chairperson of the Primary Production Committee: How many submitters on the Food Bill have requested to be heard in person? 23. Hon DAMIEN O'CONNOR to the Chairperson of the Primary Production Committee: How many submissions have been received on the Dairy Industry Restructuring (New Sunset Provisions) Amendment Bill? 24. Hon DAMIEN O'CONNOR to the Chairperson of the Primary Production Committee: How many submitters on the Dairy Industry Restructuring (New Sunset Provisions) Amendment Bill have requested to be heard in person?
Mitigating the cascade of environmental damage caused by the movement of excess reactive nitrogen (N) from land to sea is currently limited by difficulties in precisely and accurately measuring N fluxes due to variable rates of attenuation (denitrification) during transport. This thesis develops the use of the natural abundance isotopic composition of nitrate (δ15N and δ18O of NO₃-) to integrate the spatialtemporal variability inherent to denitrification, creating an empirical framework for evaluating attenuation during land to water NO₃- transfers. This technique is based on the knowledge that denitrifiers kinetically discriminate against 'heavy' forms of both N and oxygen (O), creating a parallel enrichment in isotopes of both species as the reaction progresses. This discrimination can be quantitatively related to NO₃- attenuation by isotopic enrichment factors (εdenit). However, while these principles are understood, use of NO₃- isotopes to quantify denitrification fluxes in non-marine environments has been limited by, 1) poor understanding of εdenit variability, and, 2) difficulty in distinguishing the extent of mixing of isotopically distinct sources from the imprint of denitrification. Through a combination of critical literature analysis, mathematical modelling, mesocosm to field scale experiments, and empirical studies on two river systems over distance and time, these short comings are parametrised and a template for future NO₃- isotope based attenuation measurements outlined. Published εdenit values (n = 169) are collated in the literature analysis presented in Chapter 2. By evaluating these values in the context of known controllers on the denitrification process, it is found that the magnitude of εdenit, for both δ15N and δ18O, is controlled by, 1) biology, 2) mode of transport through the denitrifying zone (diffusion v. advection), and, 3) nitrification (spatial-temporal distance between nitrification and denitrification). Based on the outcomes of this synthesis, the impact of the three factors identified as controlling εdenit are quantified in the context of freshwater systems by combining simple mathematical modelling and lab incubation studies (comparison of natural variation in biological versus physical expression). Biologically-defined εdenit, measured in sediments collected from four sites along a temperate stream and from three tropical submerged paddy fields, varied from -3‰ to -28‰ depending on the site’s antecedent carbon content. Following diffusive transport to aerobic surface water, εdenit was found to become more homogeneous, but also lower, with the strength of the effect controlled primarily by diffusive distance and the rate of denitrification in the sediments. I conclude that, given the variability in fractionation dynamics at all levels, applying a range of εdenit from -2‰ to -10‰ provides more accurate measurements of attenuation than attempting to establish a site-specific value. Applying this understanding of denitrification's fractionation dynamics, four field studies were conducted to measure denitrification/ NO₃- attenuation across diverse terrestrial → freshwater systems. The development of NO₃- isotopic signatures (i.e., the impact of nitrification, biological N fixation, and ammonia volatilisation on the isotopic 'imprint' of denitrification) were evaluated within two key agricultural regions: New Zealand grazed pastures (Chapter 4) and Philippine lowland submerged rice production (Chapter 5). By measuring the isotopic composition of soil ammonium, NO₃- and volatilised ammonia following the bovine urine deposition, it was determined that the isotopic composition of NO₃ - leached from grazed pastures is defined by the balance between nitrification and denitrification, not ammonia volatilisation. Consequently, NO₃- created within pasture systems was predicted to range from +10‰ (δ15N)and -0.9‰ (δ18O) for non-fertilised fields (N limited) to -3‰ (δ15N) and +2‰ (δ18O) for grazed fertilised fields (N saturated). Denitrification was also the dominant determinant of NO₃- signatures in the Philippine rice paddy. Using a site-specific εdenit for the paddy, N inputs versus attenuation were able to be calculated, revealing that >50% of available N in the top 10 cm of soil was denitrified during land preparation, and >80% of available N by two weeks post-transplanting. Intriguingly, this denitrification was driven by rapid NO₃- production via nitrification of newly mineralised N during land preparation activities. Building on the relevant range of εdenit established in Chapters 2 and 3, as well as the soil-zone confirmation that denitrification was the primary determinant of NO₃- isotopic composition, two long-term longitudinal river studies were conducted to assess attenuation during transport. In Chapter 6, impact and recovery dynamics in an urban stream were assessed over six months along a longitudinal impact gradient using measurements of NO₃- dual isotopes, biological populations, and stream chemistry. Within 10 days of the catastrophic Christchurch earthquake, dissolved oxygen in the lowest reaches was <1 mg l⁻¹, in-stream denitrification accelerated (attenuating 40-80% of sewage N), microbial biofilm communities changed, and several benthic invertebrate taxa disappeared. To test the strength of this method for tackling the diffuse, chronic N loading of streams in agricultural regions, two years of longitudinal measurements of NO₃- isotopes were collected. Attenuation was negatively correlated with NO₃- concentration, and was highly dependent on rainfall: 93% of calculated attenuation (20 kg NO₃--N ha⁻¹ y⁻¹) occurred within 48 h of rainfall. The results of these studies demonstrate the power of intense measurements of NO₃- stable isotope for distinguishing temporal and spatial trends in NO₃ - loss pathways, and potentially allow for improved catchment-scale management of agricultural intensification. Overall this work now provides a more cohesive understanding for expanding the use of NO₃- isotopes measurements to generate accurate understandings of the controls on N losses. This information is becoming increasingly important to predict ecosystem response to future changes, such the increasing agricultural intensity needed to meet global food demand, which is occurring synergistically with unpredictable global climate change.
Ongoing climate change triggers increasing temperature and more frequent extreme events which could limit optimal performance of haliotids, affect their physiology and biochemistry as well as influencing their population structure. Haliotids are a valuable nearshore fishery in a number of countries and many are showing a collapse of stocks because of overexploitation, environmental changes, loss of habitat, and disease. The haliotid in New Zealand commonly referred to as the blackfoot pāua (Haliotis iris) contribute a large and critical cultural, recreational and economic resource. Little was known about pāua responses to increasing temperature and acute environmental factors, as well as information about population size structure in Kaikoura after the earthquake 2016 and in Banks Peninsula. The aims of this study were to investigate the effects of temperature on scope for growth (SfG); physiological and biochemical responses of pāua subjected to different combined stressors including acute temperature, acute salinity and progressive hypoxia; and describe population size structure and shell morphology in different environments in Kaikoura and Banks Peninsula. The main findings of the present study found that population size structures of pāua were site-specific, and the shell length and shell height ratio of 3.25 could distinguish between stunted and non-stunted populations. The study found that high water temperature resulted in a reduction in absorbed energy from food, an increase in respiration energy, and ammonia excretion energy. Surveys were conducted at six study sites around the Canterbury Region over three years in order to better understand the population size structure and shell morphology of pāua. The findings found that the population size structure at 6 sites differed. Both juveniles and adults were found in intertidal areas at five sites. However, at Cape Three Points, pāua were found only in subtidal zones. One of the sites, Little Port Cooper, had a stunted population where only two pāua reached 125 mm in length over three years. In addition, most pāua in Little Port Cooper and Cape Three Points were adults, while Seal Reef had mostly juveniles. Wakatu Quay and Omihi had a full size range of pāua. Oaro population was dominated with juveniles and sub-adults. Recruitment and growth of pāua were successful after the earthquake in 2016. Research into pāua shell morphologies also determined that shell dimensions differed between sites. The relationships of shell length to shell width were linear and the relationship of shell length to shell height was curvilinear. Interestingly, SL:SH ratio of 3.25 is able to be used to identify stunted and non-stunted populations for pāua larger than 90 mm in length. Little Port Cooper was a stunted population with mean SL:SH ratio being 3.16. In the laboratory, scope for growth of pāua was investigated at four different temperatures of 12oC, 15oC, 18oC and 21oC over four weeks’ acclimation. The current study has found that SfG of pāua highly depended on temperature. Absorbed energy and respiration energy accounted for the highest proportion of the SfG of pāua. The respiration energy of pāua accounted for approximately 36%, 40%, 49% and 69% of the absorbed energy at 12°C, 15°C, 18°C and 21°C, respectively. The pāua at all acclimation temperatures had a positive scope for growth. The study suggested that the SfG was highest at 15°C, while the value at 21°C was the lowest. However, SfG at 18°C and 21°C decreased after 14 days of acclimation. Because of maintaining almost unchanged oxygen consumption over four weeks’ acclimation, pāua showed their poor abilities to acclimate to an increase in temperature. Therefore, they may be more vulnerable in future warming scenarios. The physiological and biochemical responses of pāua toward different combined stressors included three experiments. In terms of the acute temperature experiment, pāua were acclimated at 12oC, 15oC, 18oC or 21oC for two weeks before stepwise exposure to four temperatures of 12oC, 15oC, 18oC and 21oC every 4 hours. The acute salinity change, pāua were acclimated at 12oC, 15oC or 18oC over two weeks. Pāua were then exposed to a stepwise decrease of salinity of 2‰ every two hours from 34 – 22‰. Regarding the declining oxygen level, pāua were acclimated at 15 oC or 18oC for two weeks before exposure to one of four temperatures at 12oC, 15oC, 18oC or 21oC in one hour. After that acute progressive hypoxia was studied in closed respirometers for around six hours. The findings showed that there were interactions between combined stressors, affecting physiology of pāua (metabolism and heart rate). This suggests that environmental factors do not have a separate effect, but they also have interactions that enhance negative effects on pāua. Also, both oxygen uptake and heart rate responded quickly to temperature change and increased with rising temperature. On the other hand, oxygen uptake and heart rate decreased with reducing salinity and progressive hypoxia (before critical oxygen tension - Pcrit). Pcrit over four acute temperature exposures, ranged between 30.2 and 80.0 mmHg, depending on the exposure temperature. Acclimation temperature, combined with acute temperature, salinity or hypoxia stress affected the biochemistry of pāua. Pāua are osmoconformers so decreased salinity resulted in reducing haemolymph ionic concentration and increasing body volume. They were hypo-ionic with respect to sodium and potassium over the salinity ranges of 34 - 22‰. Haemocyanin accounts for a large pecentage of haemolymph protein, so trends of protein followed haemocyanin. Pāua tended to store oxygen in haemocyanin under extreme salinity stress at 22‰ and extreme hypoxia around 10 mmHg, rather than in oxygen transport. In conclusion, pāua at different sites had different population structures and morphologies. Pāua are sensitive to environmental stressors. They consumed more oxygen at high temperatures because they do not have thermal acclimation capacity. They are also osmoconformers with haemolymph sodium and potassium decreasing with salinity medium. Under progressive hypoxia, pāua could regulate oxygen and heart rate until Pcrit depending on temperature. Acute environmental changes also disturbed haemolyph parameters. 12°C and 15°C could be in the range of optimal temperature with higher SfG and less stress when exposed to acute environmental changes. Meanwhile long term exposure to 21°C is likely to be outside of the optimal range for the pāua. With ongoing climate change, pāua populations are more vulnerable so conservation is necessary. The research contributes to improving fishery management, providing insights into different environmental stressors affecting the energy demand and physiological and biochemical responses of pāua. It also allow to predicting the growth patterns and responses of pāua to adapt to climate change.